CED253 - Industrial Microbiology Lab



Course length

200 minutes / week




Shella Permatasari Santoso

About the course

Prerequisite courses

CED204 - Industrial Microbiology

Course description and learning objectives

Student completing this course will be able to:
• Apply aseptic technique in media preparation and microbe transferring.
• Isolate microbe and identification.
• Count microbe cell.
• Determine microbial growth kinetics and enzymatic kinetics.
• Demonstrate food and beverage fermentation.

Topics include

1. Preparation of suitable cultures
2. Aseptic transfer of cultures and sterile solutions
3. Isolation of microbes
4. Gram’s staining method and its observation under microscope
5. Cell- and germ-counting methods
6. Microbial growth kinetics: effect of temperature and substrate
7. Enzymatic kinetics
8. Food and beverage fermentation


1. Waites, M. J., Morgan, N. L., Rockey, J. S., Higton, G., 2001. “Industrial Microbiology: An Introduction”, UK: Blackwell Science.
2. Goldman, E. and Green, L. H., 2009. “Practical handbook of microbiology”, 2nd ed., CRC Press.

Telp: +6231 389 3933

Fax: +6231 389 1267

Jl. Kalijudan No. 37, Surabaya 60114, East Java, Indonesia

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